2021
Ding M, Malhotra R, Ottosson T, Lundqvist M, Mebrahtu A, Brengdahl J, Gehrmann U, Bäck E, Ross-Thriepland D, Isaksson I, Magnusson B, Sachsenmeier KF, Tegel H, Hober S, Uhlén M, Mayr LM, Davies R, Rockberg J, Schiavone LH. Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells. Sci Rep. 2021 Aug 18;11(1):16767. doi: 10.1038/s41598-021-96184-z. PMID: 34408239; PMCID: PMC8373891.
Bispecific Antibody Molecule Inhibits Tumor Cell Proliferation More Efficiently Than the Two-Molecule Combination.
Volk AL, Mebrahtu A, Ko BK, Lundqvist M, Karlander M, Lee HJ, Frejd FY, Kim KT, Lee JS, Rockberg J. Drugs R D. 2021 Mar 15. doi: 10.1007/s40268-021-00339-2.
Ancestral lysosomal enzymes with increased activity harbor therapeutic potential for treatment of Hunter syndrome.
Hendrikse NM, Sandegren A, Andersson T, Blomqvist J, Makower Å, Possner D, Su C, Thalén N, Tjernberg A, Westermark U, Rockberg J, Svensson Gelius S, Syrén PO, Nordling E. iScience. 2021 Feb 6;24(3):102154. doi: 10.1016/j.isci.2021.102154. eCollection 2021 Mar 19.
Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension.
2020
Evolution from adherent to suspension: systems biology of HEK293 cell line development
Malm M, Saghaleyni R, Lundqvist M, Giudici M, Chotteau V, Field R, Varley PG, Hatton D, Grassi L, Svensson T, Nielsen J & Rockberg J Sci Rep 10, 18996 (2020). https://doi.org/10.1038/s41598-020-76137-8
Systematic use of synthetic 5′-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories.
Eisenhut P, Mebrahtu A, Moradi Barzadd M, Thalén N, Klanert G, Weinguny M, Sandegren A, Su C, Hatton D, Borth N, Rockberg J.Nucleic Acids Res. 2020 Nov 18;48(20):e119. doi: 10.1093/nar/gkaa847.
Reactive oxygen species as an initiator of toxic innate immune responses in retort to SARS-CoV-2 in an ageing population, consider N-acetylcysteine as early therapeutic intervention.
Nasi A, McArdle S, Gaudernack G, Westman G, Melief C, Rockberg J, Arens R, Kouretas D, Sjölin J, Mangsbo S.Toxicol Rep. 2020 Jun 18;7:768-771. doi: 10.1016/j.toxrep.2020.06.003. eCollection 2020.
High throughput generation of a resource of the human secretome in mammalian cells
Tegel H, Dannemeyer M, Kanje S, Sivertsson Å, Berling A, Svensson AS, Hober A, Enstedt H, Volk AL, Lundqvist M, Moradi M, Afshari D, Ekblad S, Xu LL, Westin M, Bidad F, Holmberg Schiavone L, Davies R, Mayr LM, Knight S, Göpel SO, Voldborg BG, Edfors F, Forsström B, von Feilitzen K, Zwahlen M, Rockberg J, Ottosson Takanen J, Uhlén M, Hober S. (2020) N Biotechnol. Sep 25;58:45-54.doi: 10.1016/j.nbt.2020.05.002
Low Shear Stress Increases Recombinant Protein Production and High Shear Stress Increases Apoptosis in Human Cells
Zhan C, Bidkhori G, Schwarz H, Malm M, Mebrahtu A, Field R, Sellick C, Hatton D, Varley P, Mardinoglu A, Rockberg J, Chotteau V (2020) . iScience. Nov; 23(11). doi: 10.1016/j.isci.2020.101653
Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin
Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. (2020). J Biotechnol. Feb 10;309:44-52. doi: 10.1016/j.jbiotec.2019.12.017. Epub 2019 Dec 28.PMID: 31891733
2019
The human secretome.
Uhlén M, Karlsson MJ, Hober A, Svensson AS, Scheffel J, Kotol D, Zhong W, Tebani A, Strandberg L, Edfors F, Sjöstedt E, Mulder J, Mardinoglu A, Berling A, Ekblad S, Dannemeyer M, Kanje S, Rockberg J, Lundqvist M, Malm M, Volk AL, Nilsson P, Månberg A, Dodig-Crnkovic T, Pin E, Zwahlen M, Oksvold P, von Feilitzen K, Häussler RS, Hong MG, Lindskog C, Ponten F, Katona B, Vuu J, Lindström E, Nielsen J, Robinson J, Ayoglu B, Mahdessian D, Sullivan D, Thul P, Danielsson F, Stadler C, Lundberg E, Bergström G, Gummesson A, Voldborg BG, Tegel H, Hober S, Forsström B, Schwenk JM, Fagerberg L, Sivertsson Å. (2019). Sci Signal. 12(609). 10.1126/scisignal.aaz0274.
Systematic Development of Sandwich Immunoassays for the Plasma Secretome.
Häussler RS, Bendes A, Iglesias M, Sanchez-Rivera L, Dodig-Crnković T, Byström S, Fredolini C, Birgersson E, Dale M, Edfors F, Fagerberg L, Rockberg J, Tegel H, Uhlén M, Qundos U, Schwenk JM. (2019). Proteomics. (15):e1900008. 10.1002/pmic.201900008.
SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping.
Hu FJ, Lundqvist M, Uhlén M, Rockberg J. (2019). Nucleic Acids res. 47(6):e34. 10.1093/nar/gkz050.
Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion.
Lundqvist M, Thalén N, Volk AL, Hansen HG, von Otter E, Nygren PÅ, Uhlen M, Rockberg J. (2019). Sci Rep. 9(1):310. 10.1038/s41598-018-36559-x.
2018
High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome.
Zamani L, Lundqvist M, Zhang Y, Aberg M, Edfors F, Bidkhori G, Lindahl A, Mie A, Mardinoglu A, Field R, Turner R, Rockberg J, Chotteau V. (2018). Biotechnol J. (10):e1800036. 10.1002/biot.201800036.
Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains.
Volk AL, Rockberg J. (2018). Methods Mol Biol. 1785:159-183. 10.1007/978-1-4939-7841-0_11.
Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.
Volk AL, Hu FJ, Rockberg J. (2018). Methods Mol Biol. 1785:141-157. 10.1007/978-1-4939-7841-0_10.
An Introduction to Epitope Mapping.
Nilvebrant J, Rockberg J. (2018). Methods Mol Biol. 1785:1-10. 10.1007/978-1-4939-7841-0_1.
Combination of phage and Gram-positive bacterial display of human antibody repertoires enables isolation of functional high affinity binders.
Hu FJ, Volk AL, Persson H, Säll A, Borrebaeck C, Uhlen M, Rockberg J. (2018). N Biotechnol. 45:80-88. 10.1016/j.nbt.2017.07.011.
2017
A subcellular map of the human proteome.
Thul PJ, Åkesson L, Wiking M, Mahdessian D, Geladaki A, Ait Blal H, Alm T, Asplund A, Björk L, Breckels LM, Bäckström A, Danielsson F, Fagerberg L, Fall J, Gatto L, Gnann C, Hober S, Hjelmare M, Johansson F, Lee S, Lindskog C, Mulder J, Mulvey CM, Nilsson P, Oksvold P, Rockberg J, Schutten R, Schwenk JM, Sivertsson Å, Sjöstedt E, Skogs M, Stadler C, Sullivan DP, Tegel H, Winsnes C, Zhang C, Zwahlen M, Mardinoglu A, Pontén F, von Feilitzen K, Lilley KS, Uhlén M, Lundberg E. (2017). Science. 356(6340). 10.1126/science.aal3321.
Machine learning in computational biology to accelerate high-throughput protein expression.
Sastry A, Monk J, Tegel H, Uhlen M, Palsson BO, Rockberg J, Brunk E. (2017). Bioinformatics. 33(16):2487-2495. 10.1093/bioinformatics/btx207.
2016
Stratification of responders towards eculizumab using a structural epitope mapping strategy.
Volk AL, Hu FJ, Berglund MM, Nordling E, Strömberg P, Uhlen M, Rockberg J. (2016). Sci Rep. 6:31365. 10.1038/srep31365.
2015
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.
Hansen HG, Nilsson CN, Lund AM, Kol S, Grav LM, Lundqvist M, Rockberg J, Lee GM, Andersen MR, Kildegaard HF. (2015). Sci Rep. 5:18016. 10.1038/srep18016.
Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production.
Anfelt J, Kaczmarzyk D, Shabestary K, Renberg B, Rockberg J, Nielsen J, Uhlén M, Hudson EP. (2015). Microb Cell Fact. 14:167. 10.1186/s12934-015-0355-9.
Dissecting antibodies with regards to linear and conformational epitopes.
Forsström B, Axnäs BB, Rockberg J, Danielsson H, Bohlin A, Uhlen M. (2015). PLoS One. 10(3):e0121673. 10.1371/journal.pone.0121673.
Solid-phase cloning for high-throughput assembly of single and multiple DNA parts.
Lundqvist M, Edfors F, Sivertsson Å, Hallström BM, Hudson EP, Tegel H, Holmberg A, Uhlén M, Rockberg J. (2015). Nucleic Acids Res. 43(7):e49. 10.1093/nar/gkv036.
Proteomics. Tissue-based map of the human proteome.
Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, Olsson I, Edlund K, Lundberg E, Navani S, Szigyarto CA, Odeberg J, Djureinovic D, Takanen JO, Hober S, Alm T, Edqvist PH, Berling H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk JM, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Pontén F. (2015). Science. 347(6220):1260419. 10.1126/science.1260419
Combination of novel HER2-targeting antibody 1E11 with trastuzumab shows synergistic antitumor activity in HER2-positive gastric cancer.
Ko BK, Lee SY, Lee YH, Hwang IS, Persson H, Rockberg J, Borrebaeck C, Park D, Kim KT, Uhlen M, Lee JS. (2015). Mol Oncol. 9(2):398-408. 10.1016/j.molonc.2014.09.007.
2014
Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.
Forsström B, Axnäs BB, Stengele KP, Bühler J, Albert TJ, Richmond TA, Hu FJ, Nilsson P, Hudson EP, Rockberg J, Uhlen M. (2014). Mol Cell Proteomics. 13(6):1585-97. 10.1074/mcp.M113.033308.
Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display.
Volk AL, Hu FJ, Rockberg J. (2014). Methods Mol Biol. 1131:485-500. 10.1007/978-1-62703-992-5_29.
Generation of HER2 monoclonal antibodies using epitopes of a rabbit polyclonal antibody.
Hu FJ, Uhlen M, Rockberg J. (2014). N Biotechnol. 31(1):35-43. 10.1016/j.nbt.2013.10.002.
2013
Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).
Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M. (2013). J Proteome Res. 12(6):2439-48. 10.1021/pr300924j.
2012
Parallel immunizations of rabbits using the same antigen yield antibodies with similar, but not identical, epitopes.
Hjelm B, Forsström B, Löfblom J, Rockberg J, Uhlén M. (2012). PLoS One. 7(12):e45817. 10.1371/journal.pone.0045817.
Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes.
Hudson EP, Uhlen M, Rockberg J. (2012). Sci Rep. 2:706. 10.1038/srep00706.
High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays.
Buus S, Rockberg J, Forsström B, Nilsson P, Uhlen M, Schafer-Nielsen C. (2012). Mol Cell Proteomics. 11(12):1790-800. 10.1074/mcp.M112.020800.
Automated solid-phase subcloning based on beads brought into proximity by magnetic force.
Hudson EP, Nikoshkov A, Uhlen M, Rockberg J. (2012). PLoS One. 7(5):e37429. 10.1371/journal.pone.0037429.
2011
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.
Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk JM, Nilsson P, Johansson C, Uhlén M. (2011). Protein Sci. 20(11):1824-35. 10.1002/pro.716.
2010
Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies.
Kronqvist N, Malm M, Rockberg J, Hjelm B, Uhlén M, Ståhl S, Löfblom J. (2010). Recent Pat Biotechnol. 4(3):171-82. 10.2174/187220810793611536.
Epitope mapping using gram-positive surface display.
Rockberg J, Löfblom J, Hjelm B, Ståhl S, Uhlén M. (2010). Curr Protoc Immunol. Chapter 9:Unit9.9. 10.1002/0471142735.im0909s90.
Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase.
Hjelm B, Fernández CD, Löfblom J, Ståhl S, Johannesson H, Rockberg J, Uhlén M. (2010). N Biotechnol. 27(2):129-37. 10.1016/j.nbt.2009.11.001.
2009
Prediction of antibody response using recombinant human protein fragments as antigen.
Rockberg J, Uhlén M. (2009). Protein Sci. 18(11):2346-55. 10.1002/pro.245.
Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies.
Rockberg J, Schwenk JM, Uhlén M. (2009). Mol Oncol. 3(3):238-47. 10.1016/j.molonc.2009.01.003.
2008
Epitope mapping of antibodies using bacterial surface display.
Rockberg J, Löfblom J, Hjelm B, Uhlén M, Ståhl S. (2008). Nat Methods. 5(12):1039-45. 10.1038/nmeth.
A whole-genome bioinformatics approach to selection of antigens for systematic antibody generation.
Berglund L, Björling E, Jonasson K, Rockberg J, Fagerberg L, Al-Khalili Szigyarto C, Sivertsson A, Uhlén M. (2008). Proteomics. 8(14):2832-9. 10.1002/pmic.200800203.
2005
A human protein atlas for normal and cancer tissues based on antibody proteomics.
Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F. (2005). Mol Cell Proteomics. 4(12):1920-32. 10.1074/mcp.M500279-MCP200.
Selection of protein epitopes for antibody production.
Lindskog M, Rockberg J, Uhlén M, Sterky F. (2005). Biotechniques. 38(5):723-7. 10.2144/05385ST02.