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We are experts in cell biology, molecular biotechnology and systemsbiology with a strong focus on development of methods to answer questions related to Protein, Virus and Cell line engineering.

Our projects are on one side centered around discovery of gene therapy vehicles, such as AAV and novel bi-specific protein therapeutics, e.g. modulating our immune system for cancer or autoimmune diseases, while on the other side focused on the generation of mammalian cell factories and processes for an efficient production of these AAV- and protein-based biopharmaceuticals. 

We are located at KTH Royal Institute of Technology in Stockholm Sweden at AlbaNova University Center.

Research areas

 

 

Group members

Johan Rockberg
PI, Professor

I am  particularly interested in applying my expertise within protein and cell line engineering to enable a faster drug development of advanced biologics. With the mission to integrate as many steps of the drug development chain to expediate time and cost to patient, my team consists of technology experts in both the discovery and bioproduction aspects, supported with strong indication specific clinical expertise per project. 

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Magdalena Malm
Researcher, Lab Manager and Co-Supervisor

I hold a PhD in molecular biotechnology and have special expertise in the areas of protein- and cell line engineering. My main research focus is about improving therapeutic bioproduction of challenging proteins and viral vectors in mammalian cells by cell line- and vector engineering. My projects mainly revolve around understanding and increasing the secretory capacity of the human HEK293 cell line.

Magnus Lundqvist
Researcher, co-supervisor

My main focus area is transcriptomics analyzis of mammalian production systems. I am also developing tools to analyze deep-sequencing data from directed evolution experiments and Kozane, a web application for site-directed mutagenesis primer design.

Niklas Thalén
Postdoc

My main area of interest is the secretory pathway of mammalian cells. In my research, I utilize transcriptomic data as a path to engineer cell lines for improvements on protein production and to get an insight into the cell responses that occur during productional pressure. Besides this, I also focus on the development of a mammalian cell surface display system for epitope mapping purposes.

Aman Mebrahtu
PhD Student

My work focuses primarily on the development of Bispecific antibodies and a novel Bispecific format coined AffimAbs, as potential therapeutics within the field of immunotherapy and Autoimmune diseases. Additionally a great deal of the work focuses on the processability of Bispecifics in a platform based  High throughput manner to adress inherent issues related to the production of these complex molecules.  

Mona Moradi
PhD student

My research focus mainly on mammalian cell line engineering, since these cell lines are widely used both in industrial and academics environments. One of the most commonly used mammalian cell lines, is CHO cells which are able to produce proteins with complex PTMs that are similar to those produced in humans. Despite this, CHO cells, display difficulties to express many target proteins with right post-translational modifications. Therefore the main focus of my projects is to address different bottlenecks for protein production and secretion in CHO cells. This in turn will enable the pharmaceutical companies to produce cheaper and more accessible medicine so that all patients can receive right treatment regardless of economical background. 

Num Vistbacka
M.Sc. | Research Engineer

I mainly work with mammalian cell lines with focus on optimizing and developing virus and protein production in HEK293-cells (various sub-categories) and CHO-S cells for various projects in cell and protein engineering. Alongside my main project I aid my colleagues in the research group with various analysis methods, with main expertise in Western Blot and RNA isolation.

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Maximilian Karlander
PhD student
I work with affinity proteins and AAV production for gene therapy. I have designed an Affibody phage display library and performed selections from it. In the AAV project I work with modifying the capsid for increased productivity and enhanced tropism.
Hannes Thorell
PhD student

My work mainly surrounds AAVs, where I engineer, produce and evaluate new AAV variants with the goal of improving the selectivity of the AAV. 

Jonathan Persson
M. Sc. | Cell Line Technician
As a cell line technician, my work is primarily centered around mammalian cells, more specifically CHO & HEK cells, which I utilize in the development of stable cell lines expressing a wide array of proteins and constructs. I am also currently developing an in-house workflow for the CellCelector, a high-throughput analysis instrument capable of screening, selecting, and isolating single clones to speed up mammalian cell line research and stable cell line generation.